In vitro medicinal studies on ocimum gratissimum leaves. The lcesimsms analysis of 70% ethanolic fraction of o. Houngbeme 1, 2, clement gandonou 1, 2, boniface yehouenou 3, salome d. This investigation suggested that nigerian indigenous ocimum gratissimum leaves are rich in phytochemical constituents which contributed to its medicinal uses. Phytochemical screening of the plant materials revealed some differences in the phytochemical constituents of the plants tested but showed the presence of flavonoids, alkaloids, anthraquinones, saponins. Phytochemical analysis, elemental determination and some. The present study was designed to evaluate the phytochemical screening, antimicrobial and antioxidant activities of anabasis aphylla l. Phytochemical analysis of ocimum americanum linn with special. Introduction ocimum gratissimum commonly known as scent leaf is used in tradomedical practice, throughout. The antioxidant, phytochemical and nutritional properties of acetone, methanol and aqueous extracts of the leaves of ocimum gratissimum linn were investigated to evaluate the therapeutic and nutritional potential of the leaves of this plant. Phytochemical analysis tells us how important the plants are in research. In the southern part of nigeria, it is called efirin nla by the yoruba speaking tribe. Phytochemical analysis, elemental determination and some in.
Antibacterial activity of ocimum gratissimum scent leaf on. Ocimum gratissimum is commonly used as food and health purposes. Phytochemical screening and modulation of antibiotic. The aim of this study was to do a phytochemical screening of the methanol and hexane extracts of o. Phytochemical screening and antibacterial investigation of. Comparitive phytochemical analysis of medicinal plants. Phytochemical analysis of ocimum gratissimum by lcesimsms and its antioxidant and anxiolytic effects. Studies of antimicrobial properties of different leaf extract. Phytochemical screening and evaluation of cardioprotective. Phytochemical constituent and antioxidant activity of extract from the leaves of ocimum gratissimum article pdf available in scientific research and essays 25.
Lamiaceae by gcms method shubhangi nagorao ingole department of botany, bar. Phytochemical analysis and antioxidant activities of. In the coastal area of nigeria, the plant ocimum gratissimum is used in the treatment of epilepsy, high fever and diarrhea sofowora 1993. With this intuition, in this study tribulus terrestris, ocimum sanctum, ocimum gratissimum, plumbago zeylanica, are selected. Ocimum basilicum is a herb of medium size, strong scent with smooth or velvety touch.
The aim of this study is to make a comparative analysis of the proximate, mineral and phytochemical compositions of the leaves of ocimum gratissimum, vernonia amygdalina and moringa oleifera plants found within our vicinity in nigeria. This study is the first test of change in resistance of antibiotic activity by ocimum gratissimum l. Pharmacognostic and preliminary phytochemical study of ocimum. General techniques involved in phytochemical analysis. Results and discussion the qualitative analysis of the extracts from the leaf sample of ocimum sanctum showed the presence of phytochemical constituents such as tannins, saponin, flavonoids, steroid, terpenoids.
Aromatic plants abstracts, pubmed, dukes phytochemical. Family lamiaceae commonly called basil, is a culinary herb with pungent sweet smell. Phytochemical analysis of medicinal herb ocimum sanctum. The plant, ocimum gratissimum was purchased from ogige market in nsukka, enugu state, nigeria. The antioxidant of the plant extracts were assessed against 1,1diphenyl2picrylhydrazyl dpph and 2,2azinobis3 ethylbenzothiazoline6sulfonic. Extracts from the leaves of ocimum gratissimum were investigated for phytochemical constituent and antioxidant activity. Tests for tannins, steroids, terpenoids, flavonoids and cardiac glycosides were positive in both methanolic and aqueous extracts.
This investigation suggested that nigerian indigenous ocimum gratissimum leaves are rich in phytochemical constituents which. Ethanolic extracts of the leaves of ocimum gratissimum, sida acuta, telfaria occidentalis and vernonia amygdalina were screened for secondary metabolite constituents and insecticidal activity against beans weevil acanthscelides obtectus. The extraction was done using reflux extraction method and methanol as solvent. Phytochemical analysis, elemental determination and some in vitro antibacterial activity of ocimum basilicum l. Phytochemical constituents and phar macological activities of. Phytochemical constituents and antimicrobial and grain protectant activities of clove basil ocimum gratissimum l. Pdf phytochemical constituent and antioxidant activity. Antibacterial activity of nhexane extract of ocimum. Objective behind the present study was to carry out the quantitative phytochemical analysis and evaluate 1, 1diphenyl2picrylhydrazyl dpph and nitric oxide no free radical scavenging activities of single and combined leaves extracts of gongronema latifolium gl and ocimum gratissimum og.
Studies of antimicrobial properties of different leaf. Phytochemical analysis of ocimum americanum linn with. A comparative study of antimicrobial activity and phytochemical screening of aqueous and alcoholic leaf extract of. The aim of this present work therefore is to carry out phytochemical screening and elemental analysis of the sample.
To ascertain the phytochemical components responsible for the ethno medicinal properties, a qualitative and quantitative screening of the extracts of the sampled plants was conducted. Qualitative and quantitative phytochemical screening of. Phytochemical screening, antimicrobial activities and toxicity against artemia salina leach of extracts and fractions of ocimum gratissimum linn from benin b. Phytochemical constituents and phar macological activities of sweet basilocimum. The phytochemical analysis and antibacterial activity of ocimum gratissimum scent leaf and vernonia amygdalina bitter leaf extracts was carried and it showed that the extracts had antibacterial effects on some drug resistant bacteria. This study is aimed at evaluating the bioactive compounds and antibacterial activity of leaf extract of o. Phytochemical constituents and phar macological activities. To identify the cinnamic acid derivatives of the plant, a phytochemical analysis has been undertaken affording the isolation of several cinnamic acid derivatives whose chemical structure has been established by entirely spectroscopic approach based on one. Phytochemical screening of the plant materials revealed some differences in the phytochemical constituents of the plants tested but showed the presence of flavonoids, alkaloids, anthraquinones, saponins, and tannins.
Ocimum basilicum linn lamiaceae leaf extract was investigated for its chemical and elemental contents as well as its antibiotic effects. Phytochemical analysis and mineral elements composition of. In the present chapter, an effort was made to compile the ethnomedicinal, phytochemical, and pharmacological properties of ocimum gratissimum commonly known as camphor basil or ram tulsi. Flavonoid synthesis in plants is induced by light colour spectrums at both high and low energy radiations.
The best solvent of extraction for both plants was the hot water followed by the cold water even though they were unable to liberate flavonoids from bitter leaf extracts. Phytochemical analysis of vernonia amygdalina and ocimum. Ocimum gratissimum, phytochemical, proximate analysis, antimicrobial activity. In this study we investigated the proximate, phytochemical, and antimicrobial attributes of. Phytochemical constituent and antioxidant activity of extract from the leaves of ocimum gratissimum. Gcms analysis of hydroalcoholic extract lead to identification of 10 compounds. Justification for the use of ocimum gratissimum l in herbal medicine and its interaction with disc. Phytochemical analysis is devoted to the publication of original articles concerning the development, improvement, validation andor extension of application of analytical methodology in the plant sciences.
Phytochemical properties and antibacterial activity of leaf. The result of the phytochemical screening of the aqueous ocimum basilicum extract is presented in table 1 and the mineral analysis is presented in table 2. Ocimum gratissimum, phytochemical screening, total phenolic content. Phytochemical constituent and antioxidant activity of extract from the leaves of. Herbal extracts have been reported as alternate therapies for the treatment of psychiatric disorders and its associated oxidative stress.
Phytochemical screening revealed the presence of alkaloid, flavonoid, saponin, terpenoid, steroid and sterols in the extracts of aerial parts of a. Ocimum sanctum linn also known as tulsi or holybasil is an aromatic plant. Phytochemical analysis of telfaria occidentals and ocimum. This analysis revealed that contains ocimum sanctum l. It is a herbaceous plant that belongs to family lamiaceae.
Phytochemical analysis of aqueous extract of ocimum sanctum the aqueous extract of ocimum sanctum was subjected to phytochemical analysis find out the presence and absence of phytochemical constituents. Pdf phytochemical analysis of ocimum gratissimum by lcesi. Pdf ocimum basilicum is a common herb that is known for its ornamental and therapeutic importance. The quantitative analysis on saponins, alkaloids and flavonoids recorded mean results of 3. The plant ocimum gratissimum which is a naturally available species throughout the. The quantitative analysis was not done but the qualitative analysis is studied and compared. Lcesimsms analysis of hydroalcoholic fraction of o.
Phytochemical constituents and antimicrobial and grain. Nichonwu in igbo while in the northern part of nigeria, it is called daidoga effraim, et al. While quantitative phytochemical analysis showed the presence of 2. Comparative study of proximate, mineral and phytochemical. Comparative screening of phytochemical compounds in scent leaf ocimum gratissimum and bitter leaf vernonia amygdalina extracts oladosuajayi rn 1, dienye he 1, ajayi ct 2 and erinle od 2 1 department of fisheries technology, fcfft, new bussa, niger 2 department of fisheries, university of port harcourt, niger. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection including bioscreening. Pdf phytochemical constituent and antioxidant activity of. The application of atmospheric pressure chemical ionisation liquid chromatographymass spectrometry in the chemotaxonomic study of flavonoids. Growth characteristics, phytochemicals and mineral. The phytochemical evaluation of ocimum gratissimum shows that it is rich in alkaloid, tannins, phytates, flavonoids and oligosaccharides ijeh, et al 2004. Page 4235 iajps 2017, 4 11, 4234 4242 sandeep pandey issn 23497750 the analysis of the whole plant fraction. The phytochemical analysis and the antibacterial activity of o. The aqueous soxhlet extract, which is normally used in folkloric medicine and the chloroform, nbutanol and ethyl acetate fractions were subjected to phytochemical screenings.
It is widely used in ayurveda and siddha system of medicine to cure various ailments. Page 4235 iajps 2017, 4 11, 42344242 sandeep pandey issn 23497750 the analysis of the whole plant fraction. The aim of this research was to study the pharmacognostic parameters of leaves of ocimum gratissimum l. In the coastal area of nigeria, the plant ocimum gratissimum is used in the treatment of epilepsy, high fever. The objectives of the present study were phytochemical screening and study of the effects of ethanolic extract of aerial parts of ocimum basilicum basil on cardiac functions and histopathological changes in isoproterenolinduced myocardial infarction mi. Justification for the use of ocimum gratissimum l in herbal.
General techniques involved in phytochemical analysis international journal of advanced research in chemical science ijarcs page 27 mass is packed and the top of the percolator is closed. In vitro assessment of antioxidant, phytochemical and. Pdf phytochemical constituent and antioxidant activity of extract. S 1department of plant biology university of ilorin, pmb 1515, nigeria 2department of applied biology and biotech, enugu state university, enugu state. Grown in nigeria, international journal of plant research, vol. The name, molecular weight and structure of the components of the test materials was ascertained. Phytochemical properties and antibacterial activity of. Phytochemical analysis of leaf extract of ocimum americanum l. Phytochemical analysis, toxicity and antibacterial activity of benin medicinal plants extracts used in the treatment of sexually transmitted infections associated with hivaids alban g. Phytochemical analysis of ocimum gratissimum by lcesims. Welsh onion, allium fistulosum, is a wild perennial plant used both for culinary and ornamental purposes.
Maryam azam, saba irshad institute of biochemistry and biotechnology, university of the punjab, lahore. Phytochemical screening, antimicrobial activities and. Antifungal properties and phytochemical screening of extracts of african basil ocimum gratissimum l. Telfaria occidentalis and ocimum gratissimum leaves were collected from the kadobimko market, gwarinpaabuja in october 2016. Gener all y, the species of ocimum are both cultivated herbs and shrubs and wild growing. Phytochemical screening of the leaves of ocimum gratissimum and vernonia amygdalina was revealed the presence of alkaloids, flavonoids, steroid, tannins, and caroternoid. In vitro medicinal studies on ocimum gratissimum leaves usunobun usunomena, uwadiae eseosa department of basic sciences biochemistry unit, faculty of basic and applied sciences. O ozioko, a former plant taxonomist at the herbarium section of the department of botany, university of nigeria, nsukka, nigeria.
American journal of research communication udochukwu, et al. Research article phytochemical screening and mineral. Proximate, phytochemical, and in vitro antimicrobial properties of. Phytochemical analysis of medicinal plants occurring in. Ebscohost serves thousands of libraries with premium essays, articles and other content including phytochemical analysis and mineral elements composition of ocimum basilicum obtained in jos metropolis, plateau state, nigeria. Phytochemical screening and modulation of antibiotic activity by ocimum gratissimum l. Leaf extract of ocimum gratissimum and xylopia aethiopiea were analyzed against five pathogenic organisms. The qualitative phytochemical screening and gcms analysis of ocimum sanctum leaves extracts is discussed by devendran and balasubramanian 8. In the present study we investigated the phytochemical constituents of ocimum gratissimum leaf 70% ethanolic fraction oge by lcesimsms which showed. Proximate, phytochemical, and in vitro antimicrobial. Analysis and synergistic potency of the leaf extracts of ocimum gratissimum linn.
Evaluation of phytochemical, antimicrobial activities and toxicological analysis of scent leaf ocimum gratissimum l. Phytochemical and nutrient compositions of the leaves of ocimum. Phytochemical analysis of medicinal plants occurring in local area of mardan abdul wadood 1, mehreen ghufran, syed babar jamal, muhammad naeem, ajmal khan 2, rukhsana ghaffar3. Phytochemical analysis pca is devoted to the publication of original articles concerning the development, improvement, validation andor extension of application of analytical methodology in the plant sciences.
Comparitive phytochemical analysis of medicinal plants namely. In the present study we investigated the phytochemical constituents of ocimum gratissimum leaf 70% ethanolic fraction oge by lcesimsms which showed a variety of compounds that include polyphenols, flavonoids and fatty acids. Antifungal properties and phytochemical screening of. Comparative screening of phytochemical compounds in scent. Grown in nigeria abdullahi mann department of chemistry, federal university technology, minna, p. Dpph, in vitro, minerals, phytochemicals, ocimum gratissimum 1.
Pdf phytochemical analysis of vernonia amygdalina and. Combined phytochemicals from gongronema latifolium and ocimum. Justification for the use of ocimum gratissimum l in herbal medicine and its interaction with disc antibiotics. Pdf ocimum gratissimum l is a popular medicinal plant with a wide therapeutic. The analyses were investigated in accordance with standard procedures and compared. Abdullahi mann, phytochemical constituents and antimicrobial and grain protectant activities of clove basil ocimum gratissimum l. The investigation was carried out to determine the qualitative analysis of phytochemical screening and possible chemical components ocimum sanctum l. The phytochemical analysis of the plant leaves extract to the estimation of standard biochemical methods such as peroxidase, superoxide dismutase sod, polyphenol oxidase ppo. Phytochemical analysis qualitative phytochemical screening using standard methods as described 610 were carried out. Pdf preliminary phytochemical analysis and insecticidal.
Ocimum gratissimum is a common plant in the tropics and has been used in food and medicine. Justification for the use of ocimum gratissimum l in. Its usage in food and medicine could be attributed to its phtyochemical and antimicrobial properties. Phytochemical screening and antibacterial activities of essential oil, ethanolic and methanolic extracts of ocimum basillicum l.
Macroscopic study showed that the leaves of this plant possess dark green color with characteristic aromatic odour and bitter taste. Review phytochemical constituents and phar macological activities of. Pdf phytochemical screening and mineral composition of the. In this study we investigated the proximate, phytochemical, and antimicrobial attributes of air dried leaves of o. Qualitative phytochemical screening and gcms analysis of. Phytochemical analysis of ocimum gratissimum by lcesimsms. Antibacterial activity of ocimum gratissimum scent leaf. Objective behind the present study was to carry out the quantitative phytochemical analysis and evaluate 1, 1diphenyl2picrylhydrazyl dpph and nitric oxide no free radical scavenging activities of single and combined leaves extracts of gongronema latifolium gl and ocimum gratissimumog. Pdf extracts from the leaves of ocimum gratissimum were investigated for. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection including bioscreening, extraction, separation, purification, identification and.
468 215 1388 1020 623 166 1388 1587 1625 377 436 1035 1100 1233 465 87 274 1500 610 1182 602 1615 179 434 1200 498 219 1621 812 1450 1513 1135 219 1279 624 473 884 748 298 356 222 203